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Home / SERVICES / Antibody Services / Moloclonal Antibodies
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NeoScientific is a leader in custom monoclonal antibody development. Our antibody team strives to deliver successful projects to customers in the most cost-effective way. Our monoclonal antibody service delivers on the most difficult projects. We have created antibodies for numerous applications including functional assays, immuneprecipitation, IHC, IF, flow cytometry, Western blot, and ELISA. You can review more information we have on monoclonal antibodies in our blog.
GUARANTEES:
We guarantee positive clones for monoclonal antibodies development if antigens were prepared.
SERVICES INCLUDE:
NeoScientific offers a variety of hybridoma development packages and monoclonal antibody production services. Our scientists will help you select the best service for you
NeoScientific offers a variety of hybridoma development packages and monoclonal antibody production services. Our scientists will help you select the best service for you.
PACKAGE INCLUDES:
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation
YOU WILL RECEIVE (IN 4-6 MONTHS):
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
PACKAGE INCLUDES:
- Peptide synthesis
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation
YOU WILL RECEIVE (IN 4-6 MONTHS):
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
- 5 mg of unconjugated peptide
PACKAGE INCLUDES:
- Peptide synthesis
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation
YOU WILL RECEIVE (IN 4-6 MONTHS):
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
- 5 mg of phospho-peptide
- 5 mg of unmodified peptide
PACKAGE INCLUDES:
- Immunogen preparation
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation
YOU WILL RECEIVE (IN 5-7 MONTHS):
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
Note:
1. We provide free cell line storage service for 6 months from the date of cell freezing. If customers require continued storage service after the end of six-month period, the charges are set at $60 per cell line per year.
2. If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products.
NeoScientific's standard option for monoclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.
The protocol uses animals that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).
| Procedure | Protocol Day | Description |
|---|---|---|
| Control Serum Collection | Day 0 | Pre-immune bleed (5mL per rabbit) |
| Primary Injection | Day 1 | Immunize with 0.50mg of antigen in CFA, 10 SQ sites |
| 1st Booster | Day 14 | Boost with 0.25mg of antigen in IFA, 4 SQ sites |
| 2nd Booster | Day 28 | Boost with 0.25mg of antigen in IFA, 4 SQ sites |
| Serum Collection | Day 35 | Bleed (~25mL per rabbit) |
| 3rd Booster | Day 42 | Boost with 0.25mg of antigen in IFA, 4 SQ sites |
| Serum Collection | Day 56, 58 | Two bleeds (~50mL total per rabbit) |
| ELISA Test | Day 60 | ELISA titration (results available online); |
| Purification | Optional | 6-10 mg purified antibodies |